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Test Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Somatostatin Antibody (Anti-SST) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Somatostatin Antibody (Anti-SST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Information
Research AreaTumor immunity Endocrinology Hormone metabolism
Species ReactivitySus scrofa Porcine (Pig)
Sample Typeserum, plasma and other biological fluids
Specifications
Tested ApplicationELISA
SensitivityThe minimum detectable dose of this kit is typically less than 1.24ng/mL
Detection Range3.12-200ng/mL
Assay Time2h, 30min
Detection MethodEnzyme-linked immunosorbent assay for Antibody Detection.