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Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transferrin (TRF) level in the sample or standard.;
Information
Research AreaTumor immunity Infection immunity Immune molecule
Species ReactivityCanis familiaris Canine (Dog)
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Specifications
Tested ApplicationELISA
SensitivityThe minimum detectable dose of this kit is typically less than 0.13ng/mL
Detection Range0.41-300ng/mL
Assay Time2h, 40min
Detection MethodChemiluminescent immunoassay for Antigen Detection.
Misc Information
No Data Available
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