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Test Principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Substance P (SP). A competitive inhibition reaction is launched between biotin labeled Substance P (SP) and unlabeled Substance P (SP) (Standards or samples) with the pre-coated antibody specific to Substance P (SP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Substance P (SP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Substance P (SP) level in the sample or standard.
Information
Research AreaEndocrinology Neuro science Hormone metabolism
Species ReactivityCavia (Guinea pig )
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Specifications
Tested ApplicationELISA
SensitivityThe minimum detectable dose of this kit is typically less than 5.12pg/mL
Detection Range11.72-3,000pg/mL
Assay Time2h
Detection MethodChemiluminescent immunoassay for Antigen Detection.
Misc Information
No Data Available
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