tcue3515 ELISA Kit for Insulin Like Protein 3 (INSL3)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin Like Protein 3 (INSL3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin Like Protein 3 (INSL3) and unlabeled Insulin Like Protein 3 (INSL3) (Standards or samples) with the pre-coated antibody specific to Insulin Like Protein 3 (INSL3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin Like Protein 3 (INSL3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin Like Protein 3 (INSL3) in the sample.