tcue999 ELISA Kit for 1-Desamino 8D Arginine Vasopressin (DDAVP)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1-Desamino 8D Arginine Vasopressin (DDAVP) and unlabeled 1-Desamino 8D Arginine Vasopressin (DDAVP) (Standards or samples) with the pre-coated antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample.