tcue728 ELISA Kit for Prostaglandin F2 Alpha (PGF2a)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGF2a has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGF2a analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGF2a in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGF2a in the sample.