tcue688 ELISA Kit for Delta-Sleep Inducing Peptide (dSIP)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Delta-Sleep Inducing Peptide (dSIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Delta-Sleep Inducing Peptide (dSIP) and unlabeled Delta-Sleep Inducing Peptide (dSIP) (Standards or samples) with the pre-coated antibody specific to Delta-Sleep Inducing Peptide (dSIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample.