tcue337 ELISA Kit for Complement Component 4a (C4a)

Order Now

AVAILABLE SIZES

$0.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Test Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4a (C4a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4a (C4a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4a (C4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 4a (C4a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Information

Research AreaImmune molecule
Endocrinology
Species ReactivityMus musculus (Mouse)
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Specifications

Tested ApplicationELISA
SensitivityThe minimum detectable dose of this kit is typically less than 11.9pg/mL
Detection Range31.2-2,000pg/mL
Assay Time3h
Detection MethodEnzyme-linked immunosorbent assay for Antigen Detection.

Misc Information

SwissProtB9EIU2
Get valuable resources and offers directly to your email.