tcue267 ELISA Kit for Islet Amyloid Polypeptide (IAPP)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Islet Amyloid Polypeptide (IAPP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Islet Amyloid Polypeptide (IAPP) and unlabeled Islet Amyloid Polypeptide (IAPP) (Standards or samples) with the pre-coated antibody specific to Islet Amyloid Polypeptide (IAPP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Islet Amyloid Polypeptide (IAPP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Islet Amyloid Polypeptide (IAPP) in the sample.