tcue165 ELISA Kit for Gamma-Aminobutyric Acid (gABA)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gamma-Aminobutyric Acid (gABA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gamma-Aminobutyric Acid (gABA) and unlabeled Gamma-Aminobutyric Acid (gABA) (Standards or samples) with the pre-coated antibody specific to Gamma-Aminobutyric Acid (gABA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) in the sample.