tcsc2953 Hoechst 33342 (trihydrochloride)

Hoechst 33342 trihydrochloride is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

IC50 & Target: Dye reagent^[1]


DNA Stain^[1]

In Vitro: Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI^[1].