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BioChemicals
Campathecin
tcsc1049
Campathecin
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AVAILABLE SIZES
100mg
500mg
$
86.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Campathecin is a potent DNA enzyme
topoisomerase I
inhibitor, with an
IC
50
of 679 nM.
IC50 & Target: IC50: 679 nM (topoisomerase I)
[3]
In Vitro:
[
3
H]BrCPT labeling of topoisomerase I is enhanced greatly by the presence of DNA; very little labeling of isolated topoisomerase I or isolated DNA occurrs. Even in the presence of DNA, [
3
H]BrCPT labeling of topoisomerase I is inhibited by camptothecin, suggesting that both CPT and BrCPT bind to the same site on the DNA-topoisomerase I binary complex
[1]
. With increasing concentrations of camptothecin, closed circular pRR322 DNA (form I) is converted to nicked circular DNA (form 11). This apparent nicking activitv of camptothecin required DNA topoisomerase I
[2]
.
Information
CAS No
7689-03-4
Formula
C
20
H
16
N
2
O
4
Clinical Information
clinicalinformation
Pathway
Antibody-drug Conjugate/ADC Related
Anti-infection
Apoptosis
Cell Cycle/DNA Damage
Epigenetics
Target
ADC Cytotoxin
Antibiotic
Apoptosis
Fungal
Influenza Virus
MicroRNA
Topoisomerase
Specifications
Purity / Grade
99.58%
Solubility
DMSO : 6.25 mg/mL (17.94 mM; Need ultrasonic)
Smiles
O=C1C2=C([C@@](O)(CC)C(OC2)=O)C=C(N1C3)C(C3=C4)=NC5=C4C=CC=C5
Misc Information
Storage Instruction
2-8°C, protect from light
Alternative Names
Campathecin; (S)-(+)-Camptothecin; CPT
Observed Molecular Weight
348.35
References
[1]. Luzzio MJ, et al. Synthesis and antitumor activity of novel water soluble derivatives of camptothecin as specific inhibitors of topoisomerase I. Synthesis and antitumor activity of novel water soluble derivatives of camptothecin as specific inhibitors of topoisomerase I.
[2]. Bertozzi D, et al. The natural inhibitor of DNA topoisomerase I, camptothecin, modulates HIF-1α activity by changing miR expression patterns in human cancer cells. Mol Cancer Ther. 2014;13(1):239-248.
[3]. Schön M, et al. KINK-1, a novel small-molecule inhibitor of IKKbeta, and the susceptibility of melanoma cells to antitumoral treatment. J Natl Cancer Inst. 2008;100(12):862-875..
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