tcsc0029062 SN 6

SN 6 is a selective Na⁺/Ca²⁺ exchanger (NCX) inhibitor, and inhibits ⁴⁵Ca²⁺ uptake by NCX1, NCX2, and NCX3, with IC₅₀s of 2.9, 16, and 8.6 μM, respectively.

IC50 & Target: IC50：2.9 μM (NCX1), 16 μM (NCX2), 8.6 μM (NCX3)^[1]

In Vitro: SN 6 is a selective Na⁺/Ca²⁺ exchanger inhibitor, which inhibits the initial rate of ⁴⁵Ca²⁺ uptake into NCX1, NCX2, and NCX3 transfectants with IC₅₀ values of 2.9 ± 0.12, 16 ± 1.1, and 8.6 ± 0.27 μM. SN 6 (up to 30 μM) also less potently inhibits muscarinic acetylcholine receptor, with a higher IC₅₀ of 18 μM. SN 6 (0.3-30 μM) completely inhibits the initial rate of Na⁺_i-dependent ⁴⁵Ca²⁺ uptake into Na⁺-loaded sarcolemmal vesicles in a dose dependent manner (IC₅₀, 5.3 ± 0.37 μM). SN 6 (0.3-10 μM) dose-dependently protects against the hypoxia/reoxygenation-induced LDH release in parental LLC-PK1 cells and NCX1 transfectants but not in K229Q transfectants^[1]. SN 6 (1-30 μM) suppresses the bidirectional outward and inward I_NCX in a concentration-dependent manner, with IC₅₀ values of 2.3 μM and 1.9 μM, respectively. SN 6 also inhibits bidirectional current (I_NCX) in a [Na⁺]i concentration-dependent manner, with IC₅₀ values of 3.4 μM, 2.3 μM, and 1.1 μM at 10 mM, 20 mM, and 30 mM [Na⁺]i, respectively^[2]. SN 6 inhibits hypoxia/reoxygenation-induced LDH release with an IC₅₀ value of 0.63 ± 0.15 μM in NCX1 transfectants^[3].