tcsc0022180 LYN-1604

LYN-1604 is a potent UNC-51-like kinase 1 (ULK1) agonist with an EC₅₀ of 18.94 nM.

IC50 & Target: EC50: 18.94 nM (ULK1)^[1]

In Vitro: LYN-1604 is a potent UNC-51-like kinase 1 (ULK1) agonist with an EC₅₀ of 18.94 nM in ADP-Glo^™ kinase assay. The in vitro kinase assay reveals that the LYN-1604 increases the phosphorylation of mATG13 at ser318 in wild-type ULK1 transfected HEK-293T cells. It is found that LYN-1604-treated cells display a remarkable green fluorescence with MDC staining and the autophagy ratio is increased in a dose-dependent manne. It is also found that LYN-1604 induces remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II^[1].

In Vivo: Based on the results of tumor volume and weight, it is found that LYN-1604 can significantly inhibit the growth of xenograft MDA-MB-231 cells. The body weights of mice are stable, with no obvious distinctions between LYN-1604-treated and control mice. It also reveals that LYN-1604 induces remarkable autophagy in vivo^[1].