tcee21 Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

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$495.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
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Product Description

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the OD of the samples to the standard curve.

Information

ApplicationThis ELISA kit applies to the in vitro quantitative determination of Human BDNF concentrations in serum, plasma and other biological fluids.
Research AreaMetabolism, Neuroscience, Cardiovascular, Cancer
Species ReactivityHuman
Sample TypeSerum, plasma and other biological fluids
100μL
Assay TypeSandwich

Specifications

Tested ApplicationELISA
Sensitivity18.75pg/mL
Detection Range31.25~2000pg/mL
Assay Time3.5h
Detection MethodSandwich-Ab

Misc Information

Storage InstructionAn unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the detailed information on the manual once the kit is received.
NotesSpecificity: This kit recognizes Human BDNF in samples. No significant cross-reactivity or interference between Human BDNF and analogues was observed.
Both intra-CV and inter-CV are < 10%.
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