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Primary Antibody
Anti YFP antibody
tcsa25963
Anti YFP antibody
Immunofluorescence was carried out on 4% PFA fixed HEK293 cells transfected with YFP, using Anti-YFP polyclonal antibody at a 1:50 dilution.
Immunofluorescence was carried out on 4% PFA fixed HEK293 cells transfected with YFP, using Anti-YFP polyclonal antibody at a 1:50 dilution.
Immunogold labelling was carried out on epithelium cells, which were in vivo injected with Anti-YFP polyclonal antibody expressing vector.
Western Blot was carried out on HEK293 cells transduced with YFP Ad, using Anti-YFP polyclonal antibody at a 1:2,000 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
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AVAILABLE SIZES
150μg
300µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
Goat polyclonal antibody to YFP (yellow fluorescent protein). YFP is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 514/527 nm respectively.
Information
Application
WB, IF, IHC-p, IHC-F, IEM
Species Reactivity
YFP, GFP, EGFP, Venus
Host Species
Goat
Immunogen / Amino acids
Purified recombinant GFP peptide from the jellyfish aequorea victoria produced in E. coli.
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS, 20% glycerol and 0.05% sodium azide.
Concentration
3 mg/mL
Recommended Dilution
Western blot 1:500-1:2
000
Immunofluorescence 1:50-1:1
000
Immunohistochemistry (paraffin) 1:50-1:1
000
Immunohistochemistry (frozen) 1:50-1:1
000
Misc Information
Storage Instruction
Store at -20°C, and avoid repeated freeze-thaw cycles.
Alternative Names
YFP antibody
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