Product Description
It was well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) was one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor was a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
Information
ApplicationWB, IHC
Species ReactivityBovine, Dog, Chicken, Human, Mouse, Non-Human Primate, Rat, Sheep, Xenopus, Zebrafish
Host SpeciesRabbit
Immunogen / Amino acidsSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH.
ConjugationKLH
ClonalityPolyclonal
Specifications
FormLiquid
Storage Buffer100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Recommended DilutionWB 1:1000
IHC 1:100
Misc Information
Storage InstructionStore at -20°C, and avoid repeated freeze-thaw cycles.
Alternative NamesCreb1 antibodyCreb-1 antibodyCyclic AMP-responsive element-binding protein 1 antibodyCREB-1 antibodycAMP-responsive element-binding protein 1 antibodyPhospho-CREB (Ser133) (KLH) antibody