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Primary Antibody
Anti RPL13 Antibody
tcsa20040
Anti RPL13 Antibody
Western blot analysis of extracts of various cell lines, using RPL13 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast using RPL13 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spinal cord using RPL13 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RPL13 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RPL13 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RPL13 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPL13 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPL13 antibody. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human RPL13
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
RPL13 antibody60S ribosomal protein L13 antibodyBreast basic conserved protein 1 antibodyLarge ribosomal subunit protein eL13 antibodyRPL13 antibodyBBC1 antibodyOK/SW-cl.46 antibody
SwissProt
P26373
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