tcsa19821 Anti LMNB2 Antibody

Western blot analysis of extracts of various cell lines, using LMNB2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human liver injury using LMNB2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using LMNB2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using LMNB2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using LMNB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human adenomyosis using LMNB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using LMNB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using LMNB2 antibody. Blue: DAPI for nuclear staining.
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Product Description

This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.

Information

ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human LMNB2
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications

FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Misc Information

Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesLMNB2 antibody
Lamin-B2 antibody
LMNB2 antibody
LMN2 antibody
SwissProtQ03252
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