tcsa19588 Anti TP53BP1 Antibody

Western blot analysis of extracts of A-431 cells, using TP53BP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat kidney using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spinal cord using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using TP53BP1 antibody. Blue: DAPI for nuclear staining.Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using TP53BP1 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human TP53BP1
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesTP53BP1 antibody
TP53-binding protein 1 antibody
53BP1 antibody
p53-binding protein 1 antibody
p53BP1 antibody
TP53BP1 antibody
SwissProtQ12888
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