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Primary Antibody
Anti CUL4B Antibody
tcsa18962
Anti CUL4B Antibody
Western blot analysis of extracts of various cell lines, using CUL4B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using CUL4B antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Immunofluorescence analysis of U2OS cells using CUL4B antibody.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Heat mediated
Enzymatic
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Product Description
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human CUL4B
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
CUL4B antibodyCullin-4B antibodyCUL-4B antibodyCUL4B antibodyKIAA0695 antibody
SwissProt
Q13620
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