tcsa18913 Anti HDGF Antibody

Western blot analysis of extracts of various cell lines, using HDGF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat lung using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human thyroid using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human thyroid cancer using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using HDGF Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using HDGF Antibody at dilution of 1:100 (40x lens).
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Product Description
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Information
ApplicationWB, IHC
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human HDGF
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesHDGF antibody
Hepatoma-derived growth factor antibody
HDGF antibody
High mobility group protein 1-like 2 antibody
HMG-1L2 antibody
HDGF antibody
HMG1L2 antibody
SwissProtP51858
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