tcsa18408 Anti XRCC6 Antibody

Western blot analysis of extracts of various cell lines, using XRCC6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.Immunohistochemistry of paraffin-embedded human adenomyosis using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human brain astrocytoma using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human appendicitis using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using XRCC6 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using XRCC6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat, Monkey
Host SpeciesRabbit
Immunogen / Amino acidsA synthetic peptide of human XRCC6
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesXRCC6 antibody
X-ray repair cross-complementing protein 6 antibody
XRCC6 antibody
G22P1 antibody
SwissProtP12956
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