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Primary Antibody
Anti TRAP1 Antibody
tcsa18295
Anti TRAP1 Antibody
Western blot analysis of extracts of various cell lines, using TRAP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TRAP1 antibody. Blue: DAPI for nuclear staining.
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AVAILABLE SIZES
100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
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Heat mediated
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Product Description
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human TRAP1
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
TRAP1 antibodyHeat shock protein 75 kDa antibodymitochondrial antibodyHSP 75 antibodyTNFR-associated protein 1 antibodyTumor necrosis factor type 1 receptor-associated protein antibodyTRAP-1 antibodyTRAP1 antibodyHS
SwissProt
Q12931
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