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Primary Antibody
Anti IKBKB Antibody
tcsa17134
Anti IKBKB Antibody
Western blot analysis of extracts of various cell lines, using IKBKB antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung cancer using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IKBKB antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using IKBKB antibody. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Product Description
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
A synthetic peptide of human IKBKB
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
IKBKB antibodyInhibitor of nuclear factor kappa-B kinase subunit beta antibodyI-kappa-B-kinase beta antibodyIKK-B antibodyIKK-beta antibodyIkBKB antibodyIKBKB antibodyIKKB antibody
SwissProt
O14920
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