tcsa17093 Anti HSPA9 Antibody

Western blot analysis of extracts of various cell lines, using HSPA9 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat ovary using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using HSPA9 antibody at dilution of 1:100 (20x lens).Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPA9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using HSPA9 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using HSPA9 antibody. Blue: DAPI for nuclear staining.
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Product Description
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human HSPA9
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesHSPA9 antibody
Stress-70 protein antibody
mitochondrial antibody
75 kDa glucose-regulated protein antibody
GRP-75 antibody
Heat shock 70 kDa protein 9 antibody
Mortalin antibody
MOT antibody
Peptide-binding protein 74 antib
SwissProtP38646
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