tcsa17016 Anti TriMethyl Histone Antibody

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.Immunohistochemistry of paraffin-embedded rat lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Mouse lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Rat liver using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody. Blue: DAPI for nuclear staining.Chromatin immunoprecipitation analysis extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Information

ApplicationWB, IHC, IF, IP, ChIP
Species ReactivityHuman, Mouse, Rat, Other (Wide Range)
Host SpeciesRabbit
Immunogen / Amino acidsA synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
ConjugationUnconjugated
IsotypeIgG

Specifications

FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
CHIPseq 1:50 - 1:200

Misc Information

Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesHIST3H3 antibody
Histone H3.1t antibody
H3/t antibody
H3t antibody
H3/g antibody
HIST3H3 antibody
H3FT antibody
SwissProtQ16695
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