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Primary Antibody
Anti CDK4 Antibody
tcsa16408
Anti CDK4 Antibody
Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using CDK4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDK4 Antibody at dilution of 1:100 (40x lens).
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human CDK4
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
CDK4 antibodyCyclin-dependent kinase 4 antibodyCDK4 antibody
SwissProt
P11802
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