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Primary Antibody
Anti PRKCA Antibody
tcsa15218
Anti PRKCA Antibody
Western blot analysis of extracts of various cell lines, using PRKCA antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using PRKCA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PRKCA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse intestin using PRKCA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PRKCA antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of CRC cells using PRKCA antibody.
Immunofluorescence analysis of A549 cells using PRKCA antibody.
Immunofluorescence analysis of HeLa cells using PRKCA antibody.
Immunofluorescence analysis of MCF-7 cells using PRKCA antibody.
Immunofluorescence analysis of PC-12 cells using PRKCA antibody.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Information
Application
WB, IHC, IF, IP
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human PRKCA
Conjugation
Unconjugated
Clonality
Monoclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
PRKCA antibodyProtein kinase C alpha type antibodyPKC-A antibodyPKC-alpha antibodyPRKCA antibodyPKCA antibodyPRKACA antibody
SwissProt
P17252
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