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Primary Antibody
Anti HMGB1 Antibody
tcsa15132
Anti HMGB1 Antibody
Western blot analysis of extracts of various cell lines, using HMGB1 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using HMGB1 antibody.
Immunofluorescence analysis of PC-12 cells using HMGB1 antibody.
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AVAILABLE SIZES
100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human HMGB1
Conjugation
Unconjugated
Clonality
Monoclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
HMGB1 antibodyHigh mobility group protein B1 antibodyHigh mobility group protein 1 antibodyHMG-1 antibodyHMGB1 antibodyHMG1 antibody
SwissProt
P09429
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